A Novel Combination of Herbs as Chelating Agents: An In Vitro SEM Evaluation

Abstract

Background: Wide range of adverse effects associated with synthetic endodontic irrigants has gradually diverted the dental researchers to explore the possible role of herbal products with similar beneficial effects and with minimal adverse effects. The present research hypothesised that the mixture of natural herbs as irrigants (Lemon and ritha extracts) shall be effective in thoroughly disinfecting the root canal system. Methods and material: Fifty five recently extracted single rooted teeth were gathered, disinfected and stored in sterile water until use. Tooth specimens were then decoronated to achieve a uniform length of 10mm. Working length was determined 1mm short of apex; cleaning and shaping was done using K3 XF rotary files. Tooth specimens were then randomly (computerised randomisation) allocated into groups as per the irrigation protocol: Group A- Distilled water. Group B- 17% EDTA. Group C- Combination of herbal extracts without agitation. Group D- Combination of herbal extracts supplemented with ultrasonic agitation. Group E- Combination of herbal extracts supplemented with hand agitation. Tooth specimens were then sectioned longitudinally and evaluated under SEM for removal of smear layer. Obtained scores were statistical analyzed using Chi-square test, ANOVA and Tukey’s test. Results: Group B demonstrated cleaner canal walls with statistically insignificant difference with Group D. Conclusion: Experimental irrigant proves to be an effective alternative to various synthetic irrigants used in endodontic practice especially when supplemented with ultrasonic agitation.