A Recent Research Review- Development and Validation with liquid chromatography

Abstract

Nowadays many different methods of HPLC are uses for Development of Drugs. HPLC are used to able separate, quantify and detection of drug and drug related substance that can form on manufacturing or storage of drugs. Different type of Chromatographic parameters are evaluated in order for optimize the method. A mobile phase, column, temperature of column, gradient and wavelength that are use for stability and comparability of drug and drug substance and also impurities of drugs. HPLC validation method is give all information regarding like Accuracy, precision, specificity, linearity, range and limit of detection, limit of quantification, robustness and system suitability testing as per guidelines of ICH.