Simultaneous Analysis of Mometasone Furoate, Miconazole Nitrate, and Nadifloxacin in Cream Formulation By HPTLC.

Abstract

A simple and precise High performance thin layer chromatography method was developed for the simultaneous investigation of mometasone furoate (MOM), miconazole nitrate (MIC), and nadifloxacin (NAD). This method was used to analyze three drugs in a cream formulation without the interference of excipients. HPTLC separation of the drugs was achieved using the mobile phase system containing toluene, ethyl acetate, ethanol, and formic acid (10:3:2:0.5 v/v/v/v) on a precoated aluminum plate of silica gel 60 F254 at 235 nm. Linearity was achieved over the range of 60–220, 1,200–4,400, and 600–2,200 ng/band, with mean accuracy of 99.004 ± 1.008, 99.182 ± 1.324, and 99.169 ± 1.421 for MOM, MIC, and NAD, respectively. The limits of detection (ng/band) were found to be 14.075, 326.945, and 191.611, and the limits of quantification (ng/band) were 42.653, 990.741, 580.639 for MOM, MIC, and NAD, respectively, which show the sensitivity of the method. After successful development and validation, the established method was used for the assessment of mometasone furoate, miconazole nitrate, and nadifloxacin in 3 Mix cream.