Importance of Red Blood Cell Histogram and Indices Obtained Through Automated Hematology Analyzer and Comparing it with Manual Blood Film Examination

Abstract

Background: Use of automated hematology analyzer in hematology laboratory is considered as a routine as well as an essential requirement in current scenario. The burden of anemia is high worldwide and there is increasing need to diagnose and treat this condition as it may lead to many complications on long run. Aim of the study: The present study was carried out to measure the sensitivity of red blood cell histogram and indices obtained through automated hematology analyzer and comparing it with manual blood film examination in patients of anemia. Material and methods: We have done this observational cross sectional study at Central hematology laboratory of Pathology department at tertiary care center in Gujarat. One thirty patients having anemia who have consulted different departments for variable reasons were included. Demographic and laboratory data were recorded. Analysis was done by Epi info software. Results: The study of included total 130 patients having anemia as per the WHO criteria for diagnosis of anemia. In our study we found sensitivity of histogram to be 87.6%. For microcytic hypochormic anemia, the sensitivity and specificity of red blood cell histogram and indices were 94.2% and 63.1%. For normocytic anemia, the sensitivity and specificity of red blood cell histogram and indices were 96.7%% and 49.1%. For macrocytic anemia, the sensitivity and specificity of red blood cell histogram and indices were 98.2% and 90.3%. Conclusion: The study showed that red blood cell histogram and indices obtained through automated hematology analyzers are useful in initial screening purpose and it gives overall idea about the type of anemia, but for final diagnosis and confirmation, manual blood film examination still remains the gold standard test.