Evaluation of Bacterial Contamination of Dental Unit Water Lines Before and After the Use of 1% (Lysoformin 3000) Disinfectant and Awareness of the Dental Unit Waterline Disinfection Among the Practicing Dental Surgeons of Vadodara city

Abstract

Purpose: The aim is to evaluate the bacterial contamination in dental unit waterlines (DUWLs) before and after the use of the disinfectant 1% (Lysoformin 3000) for its efficacy also assess the awareness about the same among the practicing dental surgeons of Vadodara city. Methodology: A volume of 20 ml water samples from the DUWLs from the clinics of the practicing dental surgeons of Vadodara city was collected. And were asked to answer a validated questionnaire in private. Disinfection of the waterlines was performed twice a week for 2 weeks with 1% disinfectant (Lysoformin 3000) and the samples were again collected. Qualitative and quantitative microbiological analysis was performed for both the predisinfected and postdisinfected samples. Results: Predisinfection samples showed a mean colony count of 670.35 colony forming unit (CFU)/ml which was reduced to 63.90 CFU/ml (P < 0.001) postdisinfection. Questionnaire assessment showed that 70% awareness of the participants about DUWL infections and the microorganisms associated. However, only 20% practiced any type of DUWL disinfection. Conclusion: Lysoformin 3000 can be promising to be used a DUWL disinfection solution as an easy to use and readily available solution with great antibacterial properties. However, still more long‑term studies are required to check for its efficacy in anaerobic bacteria as well as resistant species of microorganisms