Comparative Evaluation of Anticandidal Activity of Pre-Incorporated Quaternary Ammonium Compound Disinfectant Alginate with 5.25% Sodium Hypochlorite Spray Disinfectant on the Conventional Alginate: An in Vivo Study

Abstract

Context: A lot of research and literature exists regarding the methods of disinfecting alginates; however, there is still need for a infallible, quick, and standardized method, which can be used as chair side disinfectant for alginate impressions in patients with candida related denture stomatitis. Aim: An In Vivo study was undertaken to evaluate the anticandidal activity of pre-incorpo- rated quaternary ammonium compound disinfectant in the alginate with conventional alginate impression material disinfected with 5.25% sodium hypochlorite spray (1 min) in primary impressions of completely edentulous patients with denture stomatitis. Methods and material: Group 1 consisted of 10 conventional alginate (Imprint) impressions disinfected by 5.25% sodium hypochlorite spray for 1 min. Group 2 consisted of 10 preincorporated quaternary ammonium compound alginates (Jeltrate Plus). Totally, 20 swabs (10 per group) were collected from palatal concavities of primary impressions and inoculated on Sabouraud GC Agar media at 37 8C for 24–96 h. Digital colony counter was used for counting colony-forming units (CFU). Statistical analysis used: The data analysis was done using descriptive statistics and independent t-test. Results: According to p value ( p < 0.01), there was a statistical significant difference in mean values of the two groups. Group 1 showed significant anticandidal activity in comparison to Group 2. Conclusions: According to limitations of this In Vivo study, sodium hypochlorite (5.25% for 1 min) spray disinfectant is more clinically effective as compared to the pre-incorporated quaternary ammonium compound alginate for patients with denture stomatitis.